Glutathione: The Master Antioxidant Your Body Needs TRT Nation In vivo upstream factors of mouse hepatotoxic mechanism with sustained hepatic glutathione depletion: Acetaminophen metabolite erythrocyte adducts and splenic macrophage generated reactive oxygen species ScienceDirect Acetaminophen's Role in Autism and ADHD: A Mitochondrial Perspective Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Tylenol And Glutathione Invigor Medical Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.